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1/64-finals
1/32-finals
1/16-finals
1/8-finals
Draw
2023/241/64-finals
| 1 | Vedomice W | Mlada Boleslav W | 10 : 1 |
| 2 | Bye | Melnik W | |
| 4 | Bye | Hradec Kralove W | |
| 8 | Bye | FK Pardubice W | |
| 9 | Taborsko W | Mokre W | 2 : 5 |
| 10 | Bye | Ceske Budejovice W | |
| 12 | Bye | Dukla Prague W | |
| 16 | Bye | Plzen W | |
| 17 | Vlasim W | FC Jilove W | 4 : 1 |
| 18 | Bye | Pribram W | |
| 20 | Bye | FC Praha W | |
| 24 | Bye | Slavia Prague W | |
| 25 | Prachatice W | Klatovy W | 0 : 13 |
| 26 | Bye | Branik W | |
| 28 | Bye | Prague Raptors W | |
| 32 | Bye | Sparta Prague W | |
| 33 | Trebes W | Bakov nad Jizerou W | 2 : 4 |
| 34 | Bye | Prostejov W | |
| 36 | Bye | Lisen W | |
| 40 | Bye | Lok. Brno W | |
| 41 | Trebic W | Okrisky W | 7 : 1 |
| 42 | Bye | Uhersky Brod W | |
| 44 | Bye | Zlin W | |
| 48 | Bye | Slovacko W | |
| 49 | Orlova W | SK Lodenice W | 3 : 11 |
| 50 | Bye | MFK Vitkovice W | |
| 52 | Bye | Olomouc W | |
| 56 | Bye | Ostrava W | |
| 57 | Banik Most - Sous W | Karlovy Vary W | 5 : 0 |
| 58 | Bye | Bohemians 1905 W | |
| 60 | Bye | Teplice W | |
| 64 | Bye | Liberec W |
1/32-finals
| 1 | Vedomice W | Melnik W | 2 : 6 |
| 2 | Bye | Hradec Kralove W | |
| 4 | Bye | FK Pardubice W | |
| 5 | Mokre W | Ceske Budejovice W | 1 : 4 |
| 6 | Bye | Dukla Prague W | |
| 8 | Bye | Plzen W | |
| 9 | Vlasim W | Pribram W | 2 : 7 |
| 10 | Bye | FC Praha W | |
| 12 | Bye | Slavia Prague W | |
| 13 | Klatovy W | Branik W | 1 : 4 |
| 14 | Bye | Prague Raptors W | |
| 16 | Bye | Sparta Prague W | |
| 17 | Bakov nad Jizerou W | Prostejov W | 1 : 5 |
| 18 | Bye | Lisen W | |
| 20 | Bye | Lok. Brno W | |
| 21 | Trebic W | Uhersky Brod W | 0 : 7 |
| 22 | Bye | Zlin W | |
| 24 | Bye | Slovacko W | |
| 25 | SK Lodenice W | MFK Vitkovice W | 1 : 2 |
| 26 | Bye | Olomouc W | |
| 28 | Bye | Ostrava W | |
| 29 | Banik Most - Sous W | Bohemians 1905 W | 2 : 3 |
| 30 | Bye | Teplice W | |
| 32 | Bye | Liberec W |
1/16-finals
| 1 | Melnik W | Hradec Kralove W | 0 : 4 |
| 2 | Bye | FK Pardubice W | |
| 3 | Ceske Budejovice W | Dukla Prague W | 1 : 0 |
| 4 | Bye | Plzen W | |
| 5 | Pribram W | FC Praha W | 1 : 2 |
| 6 | Bye | Slavia Prague W | |
| 7 | Branik W | Prague Raptors W | 1 : 0 |
| 8 | Bye | Sparta Prague W | |
| 9 | Prostejov W | Lisen W | 1 : 5 |
| 10 | Bye | Lok. Brno W | |
| 11 | Uhersky Brod W | Zlin W | 1 : 2 |
| 12 | Bye | Slovacko W | |
| 13 | MFK Vitkovice W | Olomouc W | 0 : 1 |
| 14 | Bye | Ostrava W | |
| 15 | Bohemians 1905 W | Teplice W | 5 : 0 |
| 16 | Bye | Liberec W |
1/8-finals
| 1 | Hradec Kralove W | FK Pardubice W | 0 : 3 |
| 2 | Ceske Budejovice W | Plzen W | 0 : 7 |
| 3 | FC Praha W | Slavia Prague W | 0 : 9 |
| 4 | Branik W | Sparta Prague W | 0 : 22 |
| 5 | Lisen W | Lok. Brno W | 0 : 3 |
| 6 | Zlin W | Slovacko W | 0 : 8 |
| 7 | Olomouc W | Ostrava W | 0 : 8 |
| 8 | Bohemians 1905 W | Liberec W | 0 : 4 |
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